Isolation of Human Umbilical Arterial Smooth Muscle Cells (HUASMC)
نویسندگان
چکیده
منابع مشابه
Isolation of human umbilical arterial smooth muscle cells (HUASMC).
The human umbilical cord (UC) is a biological sample that can be easily obtained just after birth. This biological sample is, most of the time, discarded and their collection does not imply any added risk to the newborn or mother s health. Moreover no ethical concerns are raised. The UC is composed by one vein and two arteries from which both endothelial cells (ECs) and smooth muscle cells (SMC...
متن کاملArterial Smooth Muscle Cells
Heparin is a potent inhibitor of arterial smooth muscle cell (SMC) migration and proliferation in vivo and in vitro. We propose that heparin affects these SMC functions by interfering with either the expression or the activity of secreted proteases required for cell movement. We have reported that heparin selectively inhibits the expression of tissue-type plasminogen activator in SMCs during mi...
متن کاملIsolation and culture of arterial smooth muscle cells from human placenta.
A simple and economical technique was developed to isolate and culture human arterial smooth muscle cells from chorionic plate vessels. Placentas from healthy women were collected at the time of term delivery. Chorionic plate arteries were identified, excised, and cut into small pieces. An explant technique was used to grow cultures of placental arterial smooth muscle (PASM) cells. Small pieces...
متن کاملCanine Arterial Smooth Muscle Cells
Background and Purpose: Previous studies have suggested that bilirubin is a potential contributor to cerebral vasospasm. The purpose of this investigation was to determine whether bilirubin accrues in subarachnoid clot, whether its vasoconstrictive effect could involve a direct action on arterial smooth muscle cells, and, if so, whether bilirubin affects their Ca2+ uptake. Methods: Subarachnoid...
متن کاملRetinoids and arterial smooth muscle cells.
Retinoic acid (RA), the active metabolite of vitamin A, is a crucial signaling molecule during vertebrate development and plays key roles in establishing cell lineages as well as in cell differentiation and proliferation.1 RA has been shown to influence the expression of many genes (see the Table) through interactions between RA receptors (RARs) and the RA response element (RARE) located in pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2010
ISSN: 1940-087X
DOI: 10.3791/1940